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KMID : 0665220230360020093
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Korean Journal of Food and Nutrition
2023 Volume.36 No. 2 p.93 ~ p.102
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Validation of Asiaticoside as Marker Compound of Centella asiatica Juice and Extract, and Its Antioxidant Activity
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Kim Yeon-Suk
Shin Hyun-Young
Ha Eun-Ji
Koo Ja-Pyeong
Jeong Se-Bin
Kim Gaeuleh
Joung Mi-Yeun
Yu Kwang-Won
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Abstract
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Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 μg/mL and LOQ was 15.12 μg/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.
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KEYWORD
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Centella asiatica, marker compounds, asiaticoside, validation, HPLC-DAD
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